Rapid screening of MMACHC gene mutations by high‐resolution melting curve analysis
نویسندگان
چکیده
منابع مشابه
Melting curve analysis for rapid detection of topoisomerase gene mutations in Haemophilus influenzae.
We established a real-time PCR assay with melting curve analysis to rapidly genotype quinolone resistance-determining regions (QRDRs) of gyrase A and topoisomerase IV genes in Haemophilus influenzae. This assay is a useful tool for the detection of fluoroquinolone resistance and for the early detection of preexisting QRDR mutations.
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Hearing loss is a common birth defect worldwide. The GJB2, SLC26A4, MT-RNR1 and MT-TS1 genes have been reported as major pathogenic genes in nonsyndromic hearing loss. Early genetic screening is recommended to minimize the incidence of hearing loss. We hereby described a multicolor melting curve analysis (MMCA)-based assay for simultaneous detection of 12 prevalent nonsyndromic hearing loss-rel...
متن کاملRapid identification of HBB gene mutations by high-resolution melting analysis.
OBJECTIVE This study was undertaken to identify HBB gene mutation. DESIGN AND METHODS Herein we evaluated high-resolution melting analysis in the identification of HBB mutations. RESULTS We have successfully established a diagnostic strategy for identifying HBB gene mutations including c.-78A>G, c.-79A>G, c.2T>G, c.79_80insT, c.84_85insC, c.123_124insT, c.125_128delTCTT, c.130 G>T, c.170G>A...
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A rapid competitive PCR method was developed to quantify DNA on the LightCycler. It rests on the quantitative information contained in the melting curves obtained after amplification in the presence of SYBR Green I. Specific hybridization probes are not required. Heterologous internal standards sharing the same primer binding sites and having different melting temperatures to the natural PCR pr...
متن کاملRapid and Accurate detection of Pseudomonas aeruginosa by real-time PCR with Melting Curve Analysis targeting gyrB gene
Title Rapid and accurate detection of Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrB gene. Author(s) Motoshima, Maiko; Yanagihara, Katsunori; Fukushima, Kazuko; Matsuda, Junichi; Sugahara, Kazuyuki; Hirakata, Yochi; Yamada, Yasuaki; Kohno, Shigeru; Kamihira, Shimeru Citation Diagnostic microbiology and infectious disease, 58(1), pp.53-58...
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ژورنال
عنوان ژورنال: Molecular Genetics & Genomic Medicine
سال: 2020
ISSN: 2324-9269,2324-9269
DOI: 10.1002/mgg3.1221